|
| Status |
Public on Oct 24, 2008 |
| Title |
hESC-derived cardiac precursors, day 40 of differentiation, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Differentiated cardiac precursor, day 40
|
| Organism |
Homo sapiens |
| Characteristics |
Differentiated H9 human embryonic stem cells, day 40, cardiomyocytes, alpha_MHC-puromyocin resistant (puromyocin selected).
|
| Treatment protocol |
H9 ESCs were transduced with a lentiviral preparation that encoded two drug selection cassettes from the same construct: a neomycin-resistance gene under the control of the REX-1 promoter (Rex-neo_resistance) and a puromycin-resistance gene under the control of the alpha-MHC promoter (aMHC-puro_resistance). Stable clonal lines were created through subsequent neo selection of a homogenous population of undifferentiated hESCs (day 0) and then differentiated for 40 days with selection of cardiac precursors using puromyocin selection.
|
| Growth protocol |
Differentiated H9 ESCs for 40 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was fractionated with the Invitrogen Purelink MicroRNA Isolation kit (selecting for >200nt fraction) following Trizol/chloroform extraction.
|
| Label |
biotin
|
| Label protocol |
According to manufacturer's (Affymetrix) recommendations.
|
| |
|
| Hybridization protocol |
According to manufacturer's (Affymetrix) recommendations.
|
| Scan protocol |
According to manufacturer's (Affymetrix) recommendations.
|
| Description |
Raw probeset RMA expression values for all probesets from H9 human embryonic stem cells differentiated to day 40 cardiomyocyte precursors.
Biological replicate 2 of 3.
|
| Data processing |
Probeset intensity values for the Affymetrix Human Exon 1.0 ST CEL files were obtained using the RMA algorithm in the Affymetrix ExpressionConsole application.
|
| |
|
| Submission date |
Oct 21, 2008 |
| Last update date |
Apr 27, 2009 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE13297 |
Cardiac precursor differentiation from hESCs by lentiviral promoter drug selection |
|
