|
| Status |
Public on Oct 24, 2008 |
| Title |
Cardiac precursor differentiation from hESCs by lentiviral promoter drug selection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Several of the essential core transcriptional control elements in human embryonic stem cells (ESCs) have been identified, but the production and function of alternative isoforms in self-renewal, pluripotency and tissue lineage specification remain largely unknown.
We have modified the H9 ESC line to allow for drug selection of human pluripotent ESCs and cardiac progenitors. Exon-level microarray expression data from undifferentiated ESCs and day 40 cardiac precursors were used to identify differentially expressed and alternative splice isoforms during differentiation.
Keywords: comparison
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| |
|
| Overall design |
RNA from a homogenous population of undifferentiated hESCs (REX1-neo promoter drug selection) and differentiated day 40 cardiomyocytes (alpha MHC-puro promoter drug selection) was isolated and profiled with exon-tiling arrays.
|
| Web link |
http://www.genmapp.org/supplemental/Salomonis_2008/hESC_exon/
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| |
|
| Contributor(s) |
Salomonis N, Conklin B |
| Citation(s) |
19893621 |
| Submission date |
Oct 21, 2008 |
| Last update date |
Jul 10, 2014 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
|
| Samples (6)
|
| GSM335817 |
Rex+ H9 ESCs, day 0, biological rep1 |
| GSM335818 |
Rex+ H9 ESCs, day 0, biological rep2 |
| GSM335819 |
Rex+ H9 ESCs, day 0, biological rep3 |
| GSM335820 |
hESC-derived cardiac precursors, day 40 of differentiation, biological rep1 |
| GSM335821 |
hESC-derived cardiac precursors, day 40 of differentiation, biological rep2 |
| GSM335822 |
hESC-derived cardiac precursors, day 40 of differentiation, biological rep3 |
|
| Relations |
| BioProject |
PRJNA109509 |
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