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Status |
Public on Oct 24, 2008 |
Title |
hESC-derived cardiac precursors, day 40 of differentiation, biological rep3 |
Sample type |
RNA |
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Source name |
Differentiated cardiac precursor, day 40
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Organism |
Homo sapiens |
Characteristics |
Differentiated H9 human embryonic stem cells, day 40, cardiomyocytes, alpha_MHC-puromyocin resistant (puromyocin selected).
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Treatment protocol |
H9 ESCs were transduced with a lentiviral preparation that encoded two drug selection cassettes from the same construct: a neomycin-resistance gene under the control of the REX-1 promoter (Rex-neo_resistance) and a puromycin-resistance gene under the control of the alpha-MHC promoter (aMHC-puro_resistance). Stable clonal lines were created through subsequent neo selection of a homogenous population of undifferentiated hESCs (day 0) and then differentiated for 40 days with selection of cardiac precursors using puromyocin selection.
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Growth protocol |
Differentiated H9 ESCs for 40 days.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was fractionated with the Invitrogen Purelink MicroRNA Isolation kit (selecting for >200nt fraction) following Trizol/chloroform extraction.
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Label |
biotin
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Label protocol |
According to manufacturer's (Affymetrix) recommendations.
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Hybridization protocol |
According to manufacturer's (Affymetrix) recommendations.
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Scan protocol |
According to manufacturer's (Affymetrix) recommendations.
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Description |
Raw probeset RMA expression values for all probesets from H9 human embryonic stem cells differentiated to day 40 cardiomyocyte precursors. Biological replicate 3 of 3.
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Data processing |
Probeset intensity values for the Affymetrix Human Exon 1.0 ST CEL files were obtained using the RMA algorithm in the Affymetrix ExpressionConsole application.
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Submission date |
Oct 21, 2008 |
Last update date |
Apr 27, 2009 |
Contact name |
Nathan Salomonis |
E-mail(s) |
nathan.salomonis@cchmc.org
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Organization name |
Cincinnati Children's Hospital
|
Department |
Biomedical Informatics
|
Lab |
Nathan Salomonis
|
Street address |
3333 Burnet Avenue
|
City |
Cincinnati |
State/province |
OH |
ZIP/Postal code |
45229 |
Country |
USA |
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|
Platform ID |
GPL5188 |
Series (1) |
GSE13297 |
Cardiac precursor differentiation from hESCs by lentiviral promoter drug selection |
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