|
Status |
Public on Dec 05, 2018 |
Title |
KJ09 |
Sample type |
genomic |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood northwest india population: Kamboj (KJ)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
|
Label |
Cy3, Cy5
|
Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
|
|
Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol, dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
Description |
Genomic DNA extracted from blood was genotyped using HumanOmniExpress-24 v1.0 BeadChip (Illumina).
|
Data processing |
Image data was analyzed using GenomeStudio v2.0.4 with GT module 2.0.4 (Illumina).
|
|
|
Submission date |
Sep 07, 2018 |
Last update date |
Feb 05, 2019 |
Contact name |
Tuuli Reisberg |
Organization name |
University of Tartu
|
Street address |
Riia 23
|
City |
Tartu |
ZIP/Postal code |
51010 |
Country |
Estonia |
|
|
Platform ID |
GPL21168 |
Series (1) |
GSE119653 |
The genetic ancestry of modern Indus Valley populations from Northwest India |
|