|
Status |
Public on Dec 31, 2008 |
Title |
wt4_mem_CD4 |
Sample type |
RNA |
|
|
Source name |
wt_mem_CD4
|
Organism |
Mus musculus |
Characteristics |
C57BL/6, Female, 8-12weeks, Spleen, excised, 60min
|
Treatment protocol |
anti-CD4, anti-CD8, and anti-CD44 staining
|
Growth protocol |
RPMI+10 FCS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy plus Mini kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Affymetrix Mouse Exon Array labeling system
|
|
|
Hybridization protocol |
Affymetrix Gene Chip Fluidics station 450
|
Scan protocol |
Affymetrix GCS3000 7G
|
Description |
exon-level analysis
|
Data processing |
Exon- and gene-level data were generated using Partek Genomic Suite 6.3 software, with the defult algorithm of RMA.
|
|
|
Submission date |
Oct 31, 2008 |
Last update date |
Jan 04, 2012 |
Contact name |
Zuopeng Wu |
E-mail(s) |
zuopeng.wu@anu.edu.au
|
Phone |
+61-2-61251392
|
Organization name |
John Curtin School of Medical Research
|
Department |
Dept. of Immunology & Genetics
|
Lab |
Immunogenomics Lab
|
Street address |
Garren Rd
|
City |
Canberra |
State/province |
ACT |
ZIP/Postal code |
0200 |
Country |
Australia |
|
|
Platform ID |
GPL6193 |
Series (1) |
GSE13416 |
Memory T cell RNA rearrangement by hn RNP LL |
|
Relations |
Alternative to |
GSM338528 (gene-level analysis) |