|
| Status |
Public on Sep 22, 2010 |
| Title |
Prol_20_2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12_proliferating; Time point: 20 min; replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
Time point: 20 min; replicate 2
|
| Treatment protocol |
Cultures of proliferating and differentiated C2C12 muscle cells in 6-well dishes containing 3 ml medium were treated with 5 µg/ml actinomycin D to arrest the transcription machinery.
|
| Growth protocol |
C2C12 cells were grown in proliferation medium (DMEM with 10% fetal bovine serum (FBS), 2% Glutamax, 1% glucose and penicillin/streptomycin). Myogenic differentiation was induced by shifting to differentiation medium (DMEM with 2% FBS, 2% Glutamax, 1% glucose and penicillin/streptomycin). C2C12 cells were maintained in differentiation medium for 8 days, when long and multinucleated myotubes had formed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA was isolated with the Machery Nagel Nucleospin RNAII Kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol for exon arrays
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol for exon arrays
|
| Scan protocol |
Standard Affymetrix protocol for exon arrays
|
| Description |
C2C12_proliferating; Time point: 20 min; replicate 2
|
| Data processing |
Median polish summarization and VSN normalization
|
| |
|
| Submission date |
Jan 12, 2009 |
| Last update date |
Sep 22, 2010 |
| Contact name |
Peter A.C. 't Hoen |
| E-mail(s) |
p.a.c.hoen@lumc.nl
|
| Phone |
+31 71 5269421
|
| Fax |
+31 71 5268285
|
| URL |
http://www.humgen.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Center for Human and Clinical Genetics
|
| Street address |
PO Box 9600
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE14387 |
RNA degradation in proliferating and differentiated C2C12 muscle precursor cells analyzed on Affymetrix exon arrays |
|
