|
Status |
Public on Apr 29, 2019 |
Title |
32S: EXOME of homo sapiens, recipient liver |
Sample type |
genomic |
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Source name |
recipient liver
|
Organism |
Homo sapiens |
Characteristics |
tissue: recipient liver gender: female age: 59
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Both donor and recipient genomic DNA were extracted from liver tissues (stored at -80°C) using an AllPrep DNA/RNA Mini Kit
|
Label |
cy3, cy5
|
Label protocol |
600ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
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|
|
Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
Scan protocol |
Illumina MiSeq® System
|
Description |
Genomic DNA extracted from donor and recipient liver was genotyped using Infinium® Human Exome-12 v1.31 BeadChip.
|
Data processing |
Genomic DNA extracted from donor and recipient liver was genotyped using Infinium ® Human Exome-12 v1.2 BeadChip (Illumina).
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|
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Submission date |
Apr 19, 2019 |
Last update date |
Apr 29, 2019 |
Contact name |
Yuan Liu |
E-mail(s) |
paradisetong@163.com
|
Phone |
18930207891
|
Organization name |
Shanghai Jiaotong University school of medicine
|
Street address |
No.277 Chongqingnan Road, Huangpu Distict,Shanghai
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200080 |
Country |
China |
|
|
Platform ID |
GPL26511 |
Series (1) |
GSE130068 |
Genomewide Association Study of Tacrolimus Pharmacokinetics Identifies Novel Single Nucleotide Polymorphisms in Convalescence Phase and Stabilizing Phase of Liver function Respectively |
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