|
| Status |
Public on Apr 29, 2019 |
| Title |
104G: EXOME of homo sapiens, donor liver |
| Sample type |
genomic |
| |
|
| Source name |
donor liver
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: donor liver
gender: male
age: 53
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Both donor and recipient genomic DNA were extracted from liver tissues (stored at -80°C) using an AllPrep DNA/RNA Mini Kit
|
| Label |
cy3, cy5
|
| Label protocol |
600ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
Illumina MiSeq® System
|
| Description |
Genomic DNA extracted from donor and recipient liver was genotyped using Infinium® Human Exome-12 v1.209 BeadChip.
|
| Data processing |
Genomic DNA extracted from donor and recipient liver was genotyped using Infinium ® Human Exome-12 v1.2 BeadChip (Illumina).
|
| |
|
| Submission date |
Apr 19, 2019 |
| Last update date |
Apr 29, 2019 |
| Contact name |
Yuan Liu |
| E-mail(s) |
paradisetong@163.com
|
| Phone |
18930207891
|
| Organization name |
Shanghai Jiaotong University school of medicine
|
| Street address |
No.277 Chongqingnan Road, Huangpu Distict,Shanghai
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200080 |
| Country |
China |
| |
|
| Platform ID |
GPL26511 |
| Series (1) |
| GSE130068 |
Genomewide Association Study of Tacrolimus Pharmacokinetics Identifies Novel Single Nucleotide Polymorphisms in Convalescence Phase and Stabilizing Phase of Liver function Respectively |
|
