|
| Status |
Public on Nov 17, 2011 |
| Title |
Control_3 |
| Sample type |
RNA |
| |
|
| Source name |
liver control
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
age: 6-8 weeks
strain: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Dhx32 homozygous mutant and control were identifed by genotyping. Liver tissues were quickly removed and snap frozen in liquid nitrogen. RNA extracted by using Trizol. cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Samples were biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
liver tissue aliquot snap frozen
|
| Data processing |
Data were processed using Expression Console Software version 1.1. on core probe sets using RMA. The p values for alternatively spliced transcripts (mutant versus control) were calculated by using the algorithms of Partek Genomics Suite version 6.4 (http://www.partek.com) on core probes excluding probes with signal less than 3.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1_na26_mm9
|
| |
|
| Submission date |
Mar 05, 2009 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Mohamed Abdelhaleem |
| E-mail(s) |
mohamed.abdelhaleem@sickkids.ca
|
| Phone |
416 813 6434
|
| Fax |
416 813 6257
|
| Organization name |
Hospital for Sick Children
|
| Department |
Pediatric Laboratory Medicine
|
| Street address |
555 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1X8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE15133 |
Exon level expression data from the liver of adult control and Dhx32 homozygous mutant mice |
|
