|
Status |
Public on May 08, 2009 |
Title |
macrophage bone marrow 2hr LPS 54 |
Sample type |
RNA |
|
|
Source name |
macrophage_bone_marrow_2hr_LPS
|
Organism |
Mus musculus |
Characteristics |
cell type: macrophage bone marrow 2hr LPS
|
Treatment protocol |
RNA extraction from tissues of naïve male (except females for specific organs) C57BL/6J mice, 8-10 weeks of age
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from cell pellets using Trizol (Invitrogen, Carlsbad, CA) followed by clean up using the RNeasy kit (Qiagen, Valencia, CA, USA).
|
Label |
biotin
|
Label protocol |
2 ug total RNA was used with the GeneChip WT Sense Target Labeling Kit
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures using GCS3000 scanner
|
Description |
macrophage_bone_marrow_2hr_LPS
|
Data processing |
RMA
|
|
|
Submission date |
May 06, 2009 |
Last update date |
May 07, 2009 |
Contact name |
John R Walker |
E-mail(s) |
jwalker@gnf.org
|
Phone |
858-812-1636
|
Organization name |
Genomics Institute of the Novartis Research Foundation
|
Lab |
Genetics Core
|
Street address |
10675 John Jay Hopkins
|
City |
San Diego |
State/province |
CA |
ZIP/Postal code |
92121 |
Country |
USA |
|
|
Platform ID |
GPL6193 |
Series (1) |
|