|
| Status |
Public on May 08, 2009 |
| Title |
macrophage bone marrow 24hr hr LPS 58 |
| Sample type |
RNA |
| |
|
| Source name |
macrophage_bone_marrow_24hr_hr_LPS
|
| Organism |
Mus musculus |
| Characteristics |
cell type: macrophage bone marrow 24hr hr LPS
|
| Treatment protocol |
RNA extraction from tissues of naïve male (except females for specific organs) C57BL/6J mice, 8-10 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets using Trizol (Invitrogen, Carlsbad, CA) followed by clean up using the RNeasy kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
2 ug total RNA was used with the GeneChip WT Sense Target Labeling Kit
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures using GCS3000 scanner
|
| Description |
macrophage_bone_marrow_24hr_hr_LPS
|
| Data processing |
RMA
|
| |
|
| Submission date |
May 06, 2009 |
| Last update date |
May 07, 2009 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
|
