RNA extraction from tissues of naïve male (except females for specific organs) C57BL/6J mice, 8-10 weeks of age
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cell pellets using Trizol (Invitrogen, Carlsbad, CA) followed by clean up using the RNeasy kit (Qiagen, Valencia, CA, USA).
Label
biotin
Label protocol
2 ug total RNA was used with the GeneChip WT Sense Target Labeling Kit
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures using GCS3000 scanner
