|
| Status |
Public on Oct 15, 2021 |
| Title |
CB IL-2 n°1 |
| Sample type |
RNA |
| |
|
| Source name |
CB IL-2 n°1
|
| Organism |
Homo sapiens |
| Characteristics |
source: cord blood
cell type: transgenic T cells
culture condition: IL-2
|
| Treatment protocol |
T cells were transduced with a retroviral vector encoding an HLA-DRB1*04-restricted TCR targeting the human papillomavirus oncoprotein E7, and cultured with IL-2 or with IL-7 and IL-15.
|
| Growth protocol |
Peripheral and cord blood mononuclear cells were obtained from healthy human donors and cord blood units that were not compliant with banking standards. T cells were activated with anti-CD3/28 microbeads and expanded with IL-2 or with IL-7 and IL-15.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ten days after activation, transgenic T cells were lysed and RNA was extracted using Qiagen Rneasy Mini Kit according to manufacturer instructions.
|
| Label |
NA
|
| Label protocol |
No label is used in Nanostring
|
| |
|
| Hybridization protocol |
A hundred nanograms of total RNA were hybridized with the NanoString Technologies nCounter CAR T Characterization panel kit according to the CodeSet Hybridization Setup instructions and the nCounter SPRINT Profiler User Manual.
|
| Scan protocol |
The nCounter SPRINT Profiler was used to access to Nanostring molecular barcoding technology.
|
| Data processing |
Gene expression data were analyzed using nSolver Analysis Software (version 4.0) and nCounter Advanced Analysis Software (version 158 2.0.134).
Normalization of the data was done using the algorithm automatically performed by the nCounter Advanced Analysis Software. Only endogenous gene normalzed counts were generated by the software and used for analysis.
|
| |
|
| Submission date |
Nov 13, 2020 |
| Last update date |
Oct 15, 2021 |
| Contact name |
Chrystel Marton |
| Organization name |
UMR1098 INSERM
|
| Street address |
8 rue du Dr Jean-François-Xavier Girod
|
| City |
Besançon |
| ZIP/Postal code |
25020 |
| Country |
France |
| |
|
| Platform ID |
GPL29417 |
| Series (1) |
| GSE161483 |
Nanostring CAR T Characterization panel comparing expression in cord blood-derived transgenic T cells cultured with IL-2 or with IL-7 and IL-15, peripheral blood-derived transgenic T cells cultured with IL-2 |
|
