|
| Status |
Public on Mar 01, 2014 |
| Title |
LoVo-Li/cOHP oxaliplatin resistant |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
LoVo-Li/cOHP
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LoVo-Li/cOHP
|
| Treatment protocol |
Induced resistance to oxaliplatin with increasing concentrations. Weekly exposure of 72hrs
|
| Growth protocol |
LoVo-Li/cOHP cells were maintained in RPMI supplemented with 10% FCS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification kit (Promega Benelux, Leiden, The Netherlands) according the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
| |
|
| Channel 2 |
| Source name |
LoVo-Li
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LoVo-Li (parental)
|
| Growth protocol |
LoVo-Li cells were maintained in RPMI supplemented with 10% FCS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification kit (Promega Benelux, Leiden, The Netherlands) according the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
| |
|
| |
| Hybridization protocol |
Hybridization protocol available via www.agilent.com
|
| Scan protocol |
Slides were scanned using the Agilent microarray scanner, protocol according to Agilent.
|
| Description |
protocols according to manufacturer (Enzo Life Sciences www.agilent.com)
|
| Data processing |
Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction software (version 9.5.1.1). Raw expression data generated by the Feature Extraction software was normalised and smoothend using the R-script "NOWAVE" as described in: Smoothing waves in array CGH tumor profiles, Bioinformatics. 2009 May 1;25(9):1099-104.
|
| |
|
| Submission date |
Feb 01, 2010 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5477 |
| Series (1) |
| GSE20144 |
aCGH profiles of parental and oxaliplatin resistant colorectal cancer and ovarian cancer cell lines |
|
