|
Status |
Public on Mar 01, 2014 |
Title |
LoVo-Li |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
LoVo-Li
|
Organism |
Homo sapiens |
Characteristics |
cell line: LoVo-Li (parental)
|
Biomaterial provider |
Marie-France Poupon
|
Growth protocol |
LoVo-Li cells were maintained in RPMI supplemented with 10% FCS
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification kit (Promega Benelux, Leiden, The Netherlands) according the manufacturer’s protocol.
|
Label |
Cy3
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
|
|
Channel 2 |
Source name |
RefXX
|
Organism |
Homo sapiens |
Characteristics |
reference: pooled WBC from healthy female volunteers
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification kit (Promega Benelux, Leiden, The Netherlands) according the manufacturer’s protocol.
|
Label |
Cy5
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
|
|
|
Hybridization protocol |
Hybridization protocol available via www.agilent.com
|
Scan protocol |
Slides were scanned using the Agilent microarray scanner, protocol according to Agilent.
|
Description |
protocols according to manufacturer (Enzo Life Sciences www.agilent.com)
|
Data processing |
Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction software (version 9.5.1.1). Raw expression data generated by the Feature Extraction software was normalised and smoothend using the R-script "NOWAVE" as described in: Smoothing waves in array CGH tumor profiles, Bioinformatics. 2009 May 1;25(9):1099-104.
|
|
|
Submission date |
Feb 01, 2010 |
Last update date |
Mar 01, 2014 |
Contact name |
Daoud Sie |
E-mail(s) |
d.sie@vumc.nl
|
Phone |
+31 20 4442428
|
Organization name |
Vrije Universiteit Medical Center
|
Department |
Pathology
|
Lab |
Microarray Core Facility
|
Street address |
De Boelelaan 1117
|
City |
Amsterdam |
ZIP/Postal code |
1081 HV |
Country |
Netherlands |
|
|
Platform ID |
GPL5477 |
Series (1) |
GSE20144 |
aCGH profiles of parental and oxaliplatin resistant colorectal cancer and ovarian cancer cell lines |
|