|
| Status |
Public on Nov 15, 2010 |
| Title |
B Cell 4 |
| Sample type |
RNA |
| |
|
| Source name |
Purified B Cells from PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
ebv staus: uninfected
cell type: Purified B Cells, from negative selection of PBMCs
day post infection or extraction: 0
|
| Treatment protocol |
Infected cells were incubated with EBV for 1h at 37 C. CFSE treatment was performed as described (Quah et al, Nature Protocols 2007).
|
| Growth protocol |
Infected cells (PBMCs) were maintained in RPMI 1640 + 15% FCS + cyclosporin A + Pen/Strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD19+ B cells were purified from PBMC using the BD iMag Negative Isolation Kit (BD #558007). Total RNA was isolated via miRvana organic extraction (Ambion Inc) according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
cDNA preparation, labeling, fragmentation performed with Gene Chip cDNA synthesis/amplification kit (Affymetrix # 900673) and Exon Array Labeling kit (Affymetrix #900671)
|
| |
|
| Hybridization protocol |
Hybridization targets were prepared from totalRNA and hybridized to GeneChip HuEx 1.0 ST arrays according to standard Affymetrix (Santa Clara,CA) GeneChip HuEx 1.0 ST and GeneChip Hybridization, Wash, and Stain protocols
|
| Scan protocol |
GeneChip Scanner 3000 7G with GeneChip Fluidics Station 450
|
| Description |
Bcell4_1083_54778_HuEx-1_15916_EL-3.CEL
|
| Data processing |
Raw .CEL data was normailized and consolidated into a total .GCT metadata file via ExpressionFileCreator RMA normalization of the 12 .CEL files in GenePattern
|
| |
|
| Submission date |
Feb 04, 2010 |
| Last update date |
Oct 29, 2010 |
| Contact name |
Luftig Laboratory |
| E-mail(s) |
jpt9@duke.edu, micah.luftig@duke.edu, pavel.nikitin@gmail.com
|
| Phone |
9196683123
|
| Organization name |
Duke University
|
| Department |
MGM
|
| Lab |
Luftig Lab
|
| Street address |
220 CARL Bldg Duke U Med Center
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE20200 |
Exon-Level Expression Data from Primary B Cells, Early Proliferating EBV-infected B Cells and Lymphoblastoid Cell Lines |
|
