|
Status |
Public on Apr 29, 2024 |
Title |
Sun-7C-2 |
Sample type |
SRA |
|
|
Source name |
human renal cancer cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: 786-O phenotype: control
|
Growth protocol |
Sun-7C and Sun-7R were routinely generated (100mm3 culture dish ) on 1640 medium
|
Extracted molecule |
total RNA |
Extraction protocol |
prefrontal cortex were rharvested , flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20. Hisat2 2.0.4 mapping to reference genome count counting Genome_build: GRCh_38 Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
Apr 29, 2021 |
Last update date |
Apr 29, 2024 |
Contact name |
Jie Wang |
E-mail(s) |
16ywbao@alumni.stu.edu.cn
|
Phone |
+86 18616260080
|
Organization name |
Changhai Hospital, Naval Medical University(Second Military Medical University)
|
Department |
Department of Urology
|
Street address |
No.168, Changhai Rd., Yangpu District
|
City |
Shanghai |
ZIP/Postal code |
200433 |
Country |
China |
|
|
Platform ID |
GPL20795 |
Series (1) |
GSE173572 |
Next Generation Sequencing Facilitates Quantitative Analysis of 786-O and sunitinib resistant 786-O (7R) cell lines |
|
Relations |
BioSample |
SAMN18923259 |
SRA |
SRX10720535 |