|
| Status |
Public on Apr 29, 2024 |
| Title |
Sun-7C-3 |
| Sample type |
SRA |
| |
|
| Source name |
human renal cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 786-O
phenotype: control
|
| Growth protocol |
Sun-7C and Sun-7R were routinely generated (100mm3 culture dish ) on 1640 medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
prefrontal cortex were rharvested , flash frozen on dry ice, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20.
Hisat2 2.0.4 mapping to reference genome
count counting
Genome_build: GRCh_38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Apr 29, 2021 |
| Last update date |
Apr 29, 2024 |
| Contact name |
Jie Wang |
| E-mail(s) |
16ywbao@alumni.stu.edu.cn
|
| Phone |
+86 18616260080
|
| Organization name |
Changhai Hospital, Naval Medical University(Second Military Medical University)
|
| Department |
Department of Urology
|
| Street address |
No.168, Changhai Rd., Yangpu District
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE173572 |
Next Generation Sequencing Facilitates Quantitative Analysis of 786-O and sunitinib resistant 786-O (7R) cell lines |
|
| Relations |
| BioSample |
SAMN18923258 |
| SRA |
SRX10720536 |
