|
| Status |
Public on May 20, 2011 |
| Title |
HL60N |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
HL60 cell line without CD34 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HL60
|
| Treatment protocol |
Total DNA extracted using manufacturer's instructions
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
Promega male control DNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Promega male control DNA
|
| Treatment protocol |
Total DNA extracted using manufacturer's instructions
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Scanned on an Agilent G2505B scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Description |
HL60 cell line without CD34 cells
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization. The WACA algorithm (F Lepretre et al., NAR 2010) was finally used for CGH waves correction for fine and reliable genomics comparisons.
|
| |
|
| Submission date |
May 20, 2010 |
| Last update date |
May 20, 2011 |
| Contact name |
frédéric leprêtre |
| E-mail(s) |
frederic.lepretre@univ-lille.fr
|
| Organization name |
Lille University
|
| Department |
CHU of Lille, CBP
|
| Lab |
plateforme de génomique fonctionnelle et structurale
|
| Street address |
Boulevard du Pr Leclercq
|
| City |
lille |
| ZIP/Postal code |
59037 |
| Country |
France |
| |
|
| Platform ID |
GPL5477 |
| Series (1) |
| GSE21923 |
Genomic profiling of HL60 and U266 blood cancer cell lines |
|
