|
| Status |
Public on Aug 04, 2010 |
| Title |
dKO brain 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse Nova1/2 double knockout brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
age: E18.5
tissue: whole brain
genotype/variation: Nova1/2 double knockout
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT Sense Target Labeling Assay
|
| |
|
| Hybridization protocol |
Affymetrix WT Sense Target Labeling Assay
|
| Scan protocol |
The scanning and image acquisition was done using Affymetrix GeneChip Operating Software (GCOS) v1.4. The scanner model is GeneChip Scanner 3000 7G.
|
| Description |
mouse Nova1/2 double knockout brain
|
| Data processing |
Affymetrix Power Tools; PLIER/IterPLIER model; brain and spinalcord data were analyzed separately. In our analysis, we used the "extended probesets" for exon analysis (MoEx-1_0-st-v1.r2.dt1.mm8.extended.ps. There are 625,534 probesets defined in this file.
probe group file: MoEx-1_0-st-v1.r2.pgf
probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.extended.ps
|
| |
|
| Submission date |
Jun 03, 2010 |
| Last update date |
Sep 15, 2011 |
| Contact name |
Chaolin Zhang |
| E-mail(s) |
czhang@rockefeler.edu
|
| Organization name |
Rockefeller University
|
| Department |
Molecular Neuro-Oncology
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE22115 |
Integrative modeling defines the Nova splicing-regulatory network and its combinatorial controls: Exon array data |
|
