|
Status |
Public on Aug 04, 2010 |
Title |
WT spinalcord 2 |
Sample type |
RNA |
|
|
Source name |
mouse wild type spinalcord
|
Organism |
Mus musculus |
Characteristics |
strain: CD1 age: E18.5 tissue: spinalcord genotype/variation: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Extraction
|
Label |
biotin
|
Label protocol |
Affymetrix WT Sense Target Labeling Assay
|
|
|
Hybridization protocol |
Affymetrix WT Sense Target Labeling Assay
|
Scan protocol |
The scanning and image acquisition was done using Affymetrix GeneChip Operating Software (GCOS) v1.4. The scanner model is GeneChip Scanner 3000 7G.
|
Description |
mouse wild type spinalcord
|
Data processing |
Affymetrix Power Tools; PLIER/IterPLIER model; brain and spinalcord data were analyzed separately. In our analysis, we used the "extended probesets" for exon analysis (MoEx-1_0-st-v1.r2.dt1.mm8.extended.ps. There are 625,534 probesets defined in this file. probe group file: MoEx-1_0-st-v1.r2.pgf probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.extended.ps
|
|
|
Submission date |
Jun 03, 2010 |
Last update date |
Sep 15, 2011 |
Contact name |
Chaolin Zhang |
E-mail(s) |
czhang@rockefeler.edu
|
Organization name |
Rockefeller University
|
Department |
Molecular Neuro-Oncology
|
Street address |
1230 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL6193 |
Series (1) |
GSE22115 |
Integrative modeling defines the Nova splicing-regulatory network and its combinatorial controls: Exon array data |
|