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Status |
Public on Mar 10, 2011 |
Title |
P19_undifferentiation_rep2 |
Sample type |
RNA |
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|
Source name |
P19 cell, undifferentiation, Day 0
|
Organism |
Mus musculus |
Characteristics |
cell line: embryonic carcinoma (EC) P19 cell line agent: untreated time: Day 0 cell type: P19 cell, undifferentiation
|
Growth protocol |
cells were culture in alpha-MEM with 10% FBS under the 5% CO2 condition. cells were treated with 1 µM RA by aggregation culture for 4 days, and were cultured additional 3 days for neuright growth
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was isolated from P19 cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
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|
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Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the Kanazawa Medical University.
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Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
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Description |
Day 00
|
Data processing |
RMA expression value derived from Expression Console software; core-exon analysis program-name = Expression Console affymetrix-algorithm-name = plier-exon-core : dabg affymetrix-algorithm-version = 2.0 Probe group file used in analysis = MoEx-1_0-st-v1.r2.pgf
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Submission date |
Aug 19, 2010 |
Last update date |
Mar 10, 2011 |
Contact name |
Hitoshi Suzuki |
E-mail(s) |
suzuki-h@jaist.ac.jp
|
Organization name |
JAIST
|
Department |
CNMT
|
Lab |
Tsukahara
|
Street address |
1-1 Asahidai
|
City |
Nomi |
State/province |
Ishikawa |
ZIP/Postal code |
923-1292 |
Country |
Japan |
|
|
Platform ID |
GPL6193 |
Series (1) |
GSE23710 |
P19 neuronal cell differentiation |
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