|
| Status |
Public on Mar 10, 2011 |
| Title |
P19_neuronal_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
P19 neuronal cell, Day 7
|
| Organism |
Mus musculus |
| Characteristics |
cell line: embryonic carcinoma (EC) P19 cell line
agent: retinoic acid (RA)
time: Day 7
cell type: P19 neuronal cell
|
| Growth protocol |
cells were culture in alpha-MEM with 10% FBS under the 5% CO2 condition. cells were treated with 1 µM RA by aggregation culture for 4 days, and were cultured additional 3 days for neuright growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from P19 cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the Kanazawa Medical University.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Day 07
|
| Data processing |
RMA expression value derived from Expression Console software; core-exon analysis
program-name = Expression Console
affymetrix-algorithm-name = plier-exon-core : dabg
affymetrix-algorithm-version = 2.0
Probe group file used in analysis = MoEx-1_0-st-v1.r2.pgf
|
| |
|
| Submission date |
Aug 19, 2010 |
| Last update date |
Mar 10, 2011 |
| Contact name |
Hitoshi Suzuki |
| E-mail(s) |
suzuki-h@jaist.ac.jp
|
| Organization name |
JAIST
|
| Department |
CNMT
|
| Lab |
Tsukahara
|
| Street address |
1-1 Asahidai
|
| City |
Nomi |
| State/province |
Ishikawa |
| ZIP/Postal code |
923-1292 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE23710 |
P19 neuronal cell differentiation |
|
