|
Status |
Public on Nov 21, 2010 |
Title |
CGH: FSCS sorted CD105+ cells from case62 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
sorted cell from case62
|
Organism |
Homo sapiens |
Characteristics |
cell type: CD105+ cell from GBM tumor age: 61 y gender: female
|
Growth protocol |
GBM specimen were dissociated into single cell and then labelled with fluorochrome-labelled antibodies fro FACS sorting
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA were extracted from freshly sorted cell population by using the Picopure DNA extraction Kit, followed by phenol extraction.
|
Label |
Cy5
|
Label protocol |
standard Agilent CGH directly labellling protocol
|
|
|
Channel 2 |
Source name |
normal human genomic DNA-Roche
|
Organism |
Homo sapiens |
Characteristics |
sample type: normal human genomic DNA-Roche
|
Growth protocol |
GBM specimen were dissociated into single cell and then labelled with fluorochrome-labelled antibodies fro FACS sorting
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA were extracted from freshly sorted cell population by using the Picopure DNA extraction Kit, followed by phenol extraction.
|
Label |
Cy3
|
Label protocol |
standard Agilent CGH directly labellling protocol
|
|
|
|
Hybridization protocol |
standard Agilent CGH protocol
|
Scan protocol |
standard Agilent protocol.
|
Data processing |
standard Agilent protocol CGH_v4_10_27_aug08, Log2 ratios were normalized by Lowess against probe intensity and mean %GC of the genomic region mapped to by the probe. Segmentation of normalized log2 ratios was by Circular Binary Segmentation (CBS, R package DNAcopy)
|
|
|
Submission date |
Sep 30, 2010 |
Last update date |
Oct 06, 2010 |
Contact name |
Viviane Tabar |
E-mail(s) |
tabarv@mskcc.org
|
Phone |
212-639-8249
|
Organization name |
MSKCC
|
Department |
neurosurgery
|
Lab |
Viviane Tabar
|
Street address |
411 east 67th street
|
City |
new york |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL5477 |
Series (2) |
GSE24452 |
Genetic abnormalities in various cell subpopulations of GBM brain tumors |
GSE24558 |
GBM brain tumors |
|