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Sample GSM609087 Query DataSets for GSM609087
Status Public on Oct 21, 2010
Title mCG-PR_SLAM_7wks_rep1
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics strain: Bl/6 Taconic
genotype/variation: mCG-PR N17
tissue: Bone marrow
cell type: SLAM
time of progression: 7 wks
Extracted molecule total RNA
Extraction protocol Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA).Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
Label biotin
Label protocol Amplified cDNA was biotin labeled using the EncoreTM Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled targets were hybridized to Mouse Exon 1.0ST arrays (Affymetrix, Santa Clara, CA).
Scan protocol Gene chips were scanned using Affymetrix GeneArray scanner GS 3000.
Description 17258
Data processing Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Oct 15, 2010
Last update date Oct 20, 2010
Contact name Nobish Varghese
E-mail(s) nvarghese@path.wustl.edu
Organization name Washington University in St.Louis
Street address 4444 Forest Park Ave.
City StLouis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6193
Series (1)
GSE24728 mCG-PML-RARA alters multipotent hematopoiesis.

Data table header descriptions
ID_REF
VALUE Exon-level RMA(core) summarized intensity

Data table
ID_REF VALUE
4304927 54.26695
4304928 1513.567
4304938 29.81334
4304943 41.43404
4304945 528.597
4304949 153.6575
4304956 158.4024
4304969 47.08295
4304972 207.3973
4304978 22.18917
4304981 571.2906
4304986 31.12833
4304997 41.09646
4305011 32.25387
4305016 181.2743
4305019 233.4367
4305028 452.2932
4305034 6460.366
4305036 1072.076
4305039 16.53511

Total number of rows: 229581

Table truncated, full table size 3786 Kbytes.




Supplementary file Size Download File type/resource
GSM609087.CEL.gz 21.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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