NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM609093 Query DataSets for GSM609093
Status Public on Oct 21, 2010
Title mCG-PR_KLS_6wks_rep3
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics strain: Bl/6 Taconic
genotype/variation: mCG-PR N17
tissue: Bone marrow
cell type: KLS
time of progression: 6 wks
Extracted molecule total RNA
Extraction protocol Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA).Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
Label biotin
Label protocol Amplified cDNA was biotin labeled using the EncoreTM Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled targets were hybridized to Mouse Exon 1.0ST arrays (Affymetrix, Santa Clara, CA).
Scan protocol Gene chips were scanned using Affymetrix GeneArray scanner GS 3000.
Description 17264
Data processing Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Oct 15, 2010
Last update date Oct 20, 2010
Contact name Nobish Varghese
E-mail(s) nvarghese@path.wustl.edu
Organization name Washington University in St.Louis
Street address 4444 Forest Park Ave.
City StLouis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6193
Series (1)
GSE24728 mCG-PML-RARA alters multipotent hematopoiesis.

Data table header descriptions
ID_REF
VALUE Exon-level RMA(core) summarized intensity

Data table
ID_REF VALUE
4304927 57.77573
4304928 1470.236
4304938 707.4026
4304943 1544.435
4304945 341.0633
4304949 148.8923
4304956 142.1406
4304969 41.75348
4304972 332.56
4304978 30.38854
4304981 39.13421
4304986 37.65145
4304997 276.4899
4305011 50.56464
4305016 337.9983
4305019 363.3275
4305028 698.9637
4305034 6223.185
4305036 1118.908
4305039 8.509001

Total number of rows: 229581

Table truncated, full table size 3786 Kbytes.




Supplementary file Size Download File type/resource
GSM609093.CEL.gz 22.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap