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Sample GSM641607 Query DataSets for GSM641607
Status Public on Dec 29, 2010
Title tumor_9495, expression
Sample type RNA
 
Source name spleen, APL
Organism Mus musculus
Characteristics strain: Bl/6 Taconic
tissue: spleen
disease state: acute promyelocytic leukemia (APL)
Extracted molecule total RNA
Extraction protocol Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA). Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
Label biotin
Label protocol Amplified cDNA was biotin labeled using the Encore Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled targets were then hybridized to Mouse Exon 1.0S T arrays (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using Affymetrix GeneArray scanner GS 3000.
Data processing Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).

This analysis included core exons.
Probe-group file: MoEx-1_0-st-v1.r2.pgf
Meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Dec 16, 2010
Last update date Dec 29, 2010
Contact name Nobish Varghese
E-mail(s) nvarghese@path.wustl.edu
Organization name Washington University in St.Louis
Street address 4444 Forest Park Ave.
City StLouis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6193
Series (2)
GSE26128 Exon array data from mouse APL tumors
GSE26131 Conserved progression mutations revealed by sequencing a mouse acute promyelocytic leukemia genome

Data table header descriptions
ID_REF
VALUE Exon-level RMA signal intensity

Data table
ID_REF VALUE
4304927 33.31164
4304928 3057.558
4304938 351.7474
4304943 2228.034
4304945 446.7842
4304949 212.2731
4304956 137.07
4304969 106.7148
4304972 208.6949
4304978 22.91486
4304981 142.7442
4304986 75.64652
4304997 137.4669
4305011 42.18568
4305016 277.342
4305019 493.8334
4305028 1274.107
4305034 5476.436
4305036 1006.852
4305039 20.8283

Total number of rows: 229581

Table truncated, full table size 3786 Kbytes.




Supplementary file Size Download File type/resource
GSM641607.CEL.gz 23.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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