NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM641613 Query DataSets for GSM641613
Status Public on Dec 29, 2010
Title tumor_9492, expression
Sample type RNA
 
Source name spleen, APL
Organism Mus musculus
Characteristics strain: Bl/6 Taconic
tissue: spleen
disease state: acute promyelocytic leukemia (APL)
Extracted molecule total RNA
Extraction protocol Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA). Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
Label biotin
Label protocol Amplified cDNA was biotin labeled using the Encore Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled targets were then hybridized to Mouse Exon 1.0S T arrays (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using Affymetrix GeneArray scanner GS 3000.
Data processing Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).

This analysis included core exons.
Probe-group file: MoEx-1_0-st-v1.r2.pgf
Meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Dec 16, 2010
Last update date Dec 29, 2010
Contact name Nobish Varghese
E-mail(s) nvarghese@path.wustl.edu
Organization name Washington University in St.Louis
Street address 4444 Forest Park Ave.
City StLouis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6193
Series (2)
GSE26128 Exon array data from mouse APL tumors
GSE26131 Conserved progression mutations revealed by sequencing a mouse acute promyelocytic leukemia genome

Data table header descriptions
ID_REF
VALUE Exon-level RMA signal intensity

Data table
ID_REF VALUE
4304927 25.58265
4304928 4469.561
4304938 411.7343
4304943 1858.865
4304945 446.7747
4304949 211.5114
4304956 150.5784
4304969 59.83239
4304972 225.5739
4304978 14.75396
4304981 138.3181
4304986 67.16698
4304997 161.4742
4305011 41.4905
4305016 240.8749
4305019 610.269
4305028 1230.867
4305034 5010.991
4305036 836.1073
4305039 41.741

Total number of rows: 229581

Table truncated, full table size 3786 Kbytes.




Supplementary file Size Download File type/resource
GSM641613.CEL.gz 23.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap