|
| Status |
Public on Dec 21, 2010 |
| Title |
AtT-20-DS8793-DEX-4-hrs |
| Sample type |
RNA |
| |
|
| Source name |
mouse pituitary corticotroph, dexamethasone 4 hours
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AtT-20
term: MGI:7087
strain: LAF1
tissue: mouse pituitary corticotroph
gender: male
time: Dex 4 hours hour
|
| Biomaterial provider |
ATCC;CCL-89
|
| Treatment protocol |
Dex treated: treatment_hour with 100 nM dexamethasone. Control, untreated 0 hour.
|
| Growth protocol |
Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37ºC incubator with 5% CO2. Cells were transferred to 10% charcoal-dextran-treated, heat-inactivated fetal bovine serum for 48 hrs prior to hormone treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells either vehicle treated or treated with 100 nM hormone (dexamethasone, Dex) for 0h, 2h, 4h or 8h. 3134 and AtT-20 RNA for microarray analysis was prepared via standard manufacturer protocols (Qiagen, Valencia, Ca) using cells resuspended in Trizol reagent (Invitrogen, Carlsbad, Ca)
|
| Label |
biotin
|
| Label protocol |
RNA from untreated and dex treated cells were labeled with biotin-CTP using manufacturer's recommendations (Affymetrix, Santa Clara, Ca)
|
| |
|
| Hybridization protocol |
Hybridizations were carried out according the manufacturer (Affymetrix) protocol.
|
| Scan protocol |
Affymterix Gene Chip Scanner 3000
|
| Description |
The AtT‐20 cell line is an anterior pituitary corticotroph of murine origin. Dexamethasone treated, 4 hours.
|
| Data processing |
Probe-level CEL files were processed through Affymetrix Expression Console using RMA summarization and median normalization methods.
MoEx-1_0-st-v1.r2.pgf
|
| |
|
| Submission date |
Dec 20, 2010 |
| Last update date |
Dec 21, 2010 |
| Contact name |
John A Stamatoyannopoulos |
| E-mail(s) |
jstam@altius.org
|
| Organization name |
Altius Institute / University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Stamatoyannopoulos
|
| Street address |
2211 Elliott Avenue, 4th Floor
|
| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98121 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE26189 |
Comparison of exon profiles before and after hormone induction |
|
