|
| Status |
Public on Jun 01, 2012 |
| Title |
ES20 |
| Sample type |
RNA |
| |
|
| Source name |
mouse ES EB
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embyonic Body cell 20
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the miRNeasy Mini Kit (Qiagen), according to the manufacturers instructions. Inclusion of 10 mL/L of the RNase inhibitor NucleoGuard (AmpTec) in the lysis buffer improved RNA yield and recoveries of long transcripts. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA. A 2-round amplification was performed with the ExpressArt TRinucleotide mRNA Amplification Kit (AmpTec) according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at NIH.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; extended-exon analysis
|
| Data processing |
Raw data were processed with the Expression console (Affymetrix) for background correction and normalization log2 transformation. Data analysis and statistical evaluations were performed with customized R codes (version 2.10.1, http://www.r-project.org/).
|
| |
|
| Submission date |
Feb 17, 2011 |
| Last update date |
Jul 19, 2013 |
| Contact name |
chunjiang he |
| Organization name |
university of chicago
|
| Street address |
900 east 57th st
|
| City |
chicago |
| State/province |
il |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE27397 |
26 mouse ES EB samples affymetrix exon array (affymetrix exon array) |
|
