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| Status |
Public on Nov 01, 2011 |
| Title |
NMuMG_Ex_control |
| Sample type |
RNA |
| |
|
| Source name |
normal murine mammary gland epithelial cell line (NMMuMG), untreated
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NMuMG
tissue origin: mammary gland
|
| Treatment protocol |
Cells were treated with 1 ng/ml recombinant human TGF-beta1 (R&D Systems, Minneapolis, MN) or left untreated.
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| Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 4.5 g/l glucose, 10% fetal bovine serum (FBS), 10 ug/ml insulin, 50 U/ml penicillin, and 50 ug/ml streptomycin.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
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| Label protocol |
Labeling was performed according to the protocols provided by Affymetrix.
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| Hybridization protocol |
Hybridization was performed according to the protocols provided by Affymetrix, using GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
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| Scan protocol |
Scanning was performed according to the protocols provided by Affymetrix.
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| Description |
exon level data
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| Data processing |
CEL files were generated using Affymetrix GeneChip Command Console Software (AGCC).
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| |
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| Submission date |
Mar 25, 2011 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
|
| Organization name |
University of Tokyo
|
| Department |
Pathology
|
| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
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| Platform ID |
GPL6193 |
| Series (1) |
| GSE28184 |
Expression data from normal murine mammary epithelial cell line NMuMG stimulated with TGF-beta |
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| Relations |
| Alternative to |
GSM697690 (gene-level analysis) |
| Data table header descriptions |
| ID_REF |
|
| VALUE |
RMA singal estimates of CORE probesets from AGCC. |
| Data table |
| ID_REF |
VALUE |
| 4304927 |
19.06186 |
| 4304928 |
3050.651 |
| 4304938 |
1084.717 |
| 4304943 |
368.2787 |
| 4304945 |
1707.85 |
| 4304949 |
804.5704 |
| 4304956 |
112.4224 |
| 4304969 |
523.8204 |
| 4304972 |
339.1012 |
| 4304978 |
197.2693 |
| 4304981 |
442.6243 |
| 4304986 |
35.27538 |
| 4304997 |
336.5587 |
| 4305011 |
16.18209 |
| 4305016 |
72.62421 |
| 4305019 |
54.49018 |
| 4305028 |
1176.06 |
| 4305034 |
11521.21 |
| 4305036 |
25.39575 |
| 4305039 |
37.1868 |
Total number of rows: 229581
Table truncated, full table size 3787 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM697687_01_NMuMG_-_BMR-H2_No_1082_MoEx-1_0-st-v1_.CEL.gz |
23.1 Mb |
(ftp)(http) |
CEL |
| Processed data included within Sample table |
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