|
| Status |
Public on May 20, 2023 |
| Title |
iPSCs treated with 5 μg/mL ECR (replicate 1) |
| Sample type |
SRA |
| |
|
| Source name |
iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC
treatment: 5 ug/mL ECR
|
| Extracted molecule |
total RNA |
| Extraction protocol |
One mg of total RNA was processed for preparing mRNA sequencing library using MGIEasy RNA Directional Library Prep Kit (MGI Tech, China) according to manufacturer’s instruction.
Library was quantified by QauntiFluor ssDNA System (Promega, WI, USA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Description |
iPSCs treated with 5 μg/mL ECR (replicate 1)
N10_L_1
|
| Data processing |
Quality of raw sequence data was checked via the FastQC and adapter or low quality bases were trimmed with TrimGalore
After trimming, the reads were aligned to the human reference genome (hg38) using the STAR aligner
Read count and transcripts per million (TPM) were calculated as gene expression abundance using the RSEM software (v1.3.3) and gene annotation GRCh38.84 Gene Transfer Format (GTF) file
Assembly: hg38
Supplementary files format and content: tab-delimited text files include count values for each Sample
|
| |
|
| Submission date |
Feb 21, 2023 |
| Last update date |
May 20, 2023 |
| Contact name |
Su-Jin Baek |
| Organization name |
KOREA INSTITUTE of ORIENTAL MEDICINE
|
| Street address |
1672, Yuseong-daero
|
| City |
Daejeon |
| ZIP/Postal code |
34054 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL30209 |
| Series (1) |
| GSE225709 |
An ethanol extract of Coptidis rhizoma induces apoptotic cell death in induced pluripotent stem cells and suppress teratoma formation |
|
| Relations |
| BioSample |
SAMN33392765 |
| SRA |
SRX19452753 |
