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Sample GSM722426 Query DataSets for GSM722426
Status Public on Oct 07, 2011
Title TCF7L2_U937bio_r1_100505_7
Sample type SRA
 
Source name TCF7L2 ChIP-seq U937 BIO
Organism Homo sapiens
Characteristics cell line: U937
chip antibody: TCF7L2
antibody catalog number: Santa Cruz SC-8631
Treatment protocol Cells were stimulated for 2hrs with 5μM BIO dissolved in DMSO.
Growth protocol U937 cells were purchased from the American Type Culture Collection (ATCC), Manassas, VA. Cells were maintained under typical conditions in RPMI-1640 media with 10% Fetal Bovine Serum by the National Cell Culture Center(NCCC). For location analysis, cells were grown to a density of 1 million per ml and 99% viability prior to cross-linked with formaldehyde for 20 min.
Extracted molecule genomic DNA
Extraction protocol Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 1000 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
 
Description TCF7L2 ChIP-seq in U937 treated with BIO
Data processing Images analysis and base calling was done using the solexa pipeline.
For all samples reads were aligned to their indicated build (hg18) using Eland.
For all samples aligned sequences were extended 150bp upstream and 0bp downstream (with respect to read strand) and allocated into 10bp bins. Counts were normalized to reads per million, and bins with at least 0.5-1 reads per million are shown.
 
Submission date May 10, 2011
Last update date May 15, 2019
Contact name Teresa Venezia Bowman
Organization name Children's Hospital Boston
Lab Zon Laboratory
Street address One Blackfan Circle, 7th floor
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL9115
Series (2)
GSE29195 Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)
GSE29196 Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration
Relations
SRA SRX097098
BioSample SAMN00717430

Supplementary file Size Download File type/resource
GSM722426_TCF7L2_U937bio_r1_100505_7_HG18_eland.ylf.gz 11.3 Mb (ftp)(http) YLF
SRA Run SelectorHelp
Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA

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