|
| Status |
Public on Sep 25, 2023 |
| Title |
WT_Inf_CD11c_3 |
| Sample type |
RNA |
| |
|
| Source name |
bead-enriched CD11c+ cells from lung homogenate
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
time: day 7 post N. Brasiliensis infection
|
| Treatment protocol |
n/a
|
| Growth protocol |
Lung from WT or KO mice was homogenized into a singel cell suspension, CD11c-positive cells were bead enriched using Miltenyi MACS cell separation per manufacterers protocol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole cell lysates were directly added to the nanostring hybridization step using 5,000 cells per manufacterers protocol.
|
| Label |
N/A
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Lysates were hybridized to Nanostring Mouse Myeloid Innate Immunity probes sets overnight per the manufacterers protocol.
|
| Scan protocol |
Samples were loaded onto the nanostring cartrisge and read using the Nanostring nCounter Sprint.
|
| Description |
SAMPLE 3
50ng of total RNA was hybridized using the Mouse Immunology probeset from Nanstring which includes 561 target genes and 15 internal reference genes.
|
| Data processing |
Data was processed using Rosalind Bio, where normalization and background subtraction were performed.
|
| |
|
| Submission date |
Jun 28, 2023 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Meera Nair |
| E-mail(s) |
meera.nair@ucr.edu
|
| Phone |
9518277772
|
| Organization name |
UC Riverside
|
| Street address |
243 SOM Research 3401 Watkins Drive
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92507 |
| Country |
USA |
| |
|
| Platform ID |
GPL25266 |
| Series (2) |
| GSE236080 |
Gene expression panel analysis of CD11c+ cells from WT and RELMĪ± KO lung homogenate at day 30 post infection with Nippostrongylus brasiliensis. |
| GSE236093 |
Myeloid and epithelial-derived RELM contribute to tissue repair following lung helminth infection |
|
