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Sample GSM7518029 Query DataSets for GSM7518029
Status Public on Sep 25, 2023
Title KO_Inf_CD11c_2
Sample type RNA
 
Source name bead-enriched CD11c+ cells from lung homogenate
Organism Mus musculus
Characteristics genotype: KO: Retnla-/-,td tomato
time: day 7 post N. Brasiliensis infection
Treatment protocol n/a
Growth protocol Lung from WT or KO mice was homogenized into a singel cell suspension, CD11c-positive cells were bead enriched using Miltenyi MACS cell separation per manufacterers protocol.
Extracted molecule total RNA
Extraction protocol Whole cell lysates were directly added to the nanostring hybridization step using 5,000 cells per manufacterers protocol.
Label N/A
Label protocol n/a
 
Hybridization protocol Lysates were hybridized to Nanostring Mouse Myeloid Innate Immunity probes sets overnight per the manufacterers protocol.
Scan protocol Samples were loaded onto the nanostring cartrisge and read using the Nanostring nCounter Sprint.
Description SAMPLE 5
50ng of total RNA was hybridized using the Mouse Immunology probeset from Nanstring which includes 561 target genes and 15 internal reference genes.
Data processing Data was processed using Rosalind Bio, where normalization and background subtraction were performed.
 
Submission date Jun 28, 2023
Last update date Sep 25, 2023
Contact name Meera Nair
E-mail(s) meera.nair@ucr.edu
Phone 9518277772
Organization name UC Riverside
Street address 243 SOM Research 3401 Watkins Drive
City Riverside
State/province CA
ZIP/Postal code 92507
Country USA
 
Platform ID GPL25266
Series (2)
GSE236080 Gene expression panel analysis of CD11c+ cells from WT and RELMĪ± KO lung homogenate at day 30 post infection with Nippostrongylus brasiliensis.
GSE236093 Myeloid and epithelial-derived RELM contribute to tissue repair following lung helminth infection

Data table header descriptions
ID_REF
VALUE Raw data: Linear counts, analyzed data: normalized and background subtracted

Data table
ID_REF VALUE
2810417H13Rik 4.763115079
2900026A02Rik 0.808918768
Abcc8 1.393881269
Acad10 4.268350387
Acly 9.214060231
Acot11 4.393881269
Acot3 3.268350387
Acox1 9.48663841
Adam19 5.917443225
Adam8 9.326588156
Adamts1 4.268350387
Adamts12 1.393881269
Adamts14 1.808918768
Adamts17 -0.191081232
Adamts2 4.61627369
Adamts3 2.130846863
Adamts4 1.808918768
Adamts9 -0.191081232
Adcyap1r1 4.332480724
Adgre1 9.194781169

Total number of rows: 734

Table truncated, full table size 12 Kbytes.




Supplementary file Size Download File type/resource
GSM7518029_20180424_30102092601019-01_Sample11_11.RCC.gz 8.4 Kb (ftp)(http) RCC
Processed data included within Sample table

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