NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM759515 Query DataSets for GSM759515
Status Public on Jul 14, 2011
Title [E-MTAB-513] HCT20148, skeletal muscle, male, 77 years, 2x50 paired end mRNA-seq
Sample type SRA
 
Source name skeletal muscle, male, 77 years, 2x50 paired end mRNA-seq
Organism Homo sapiens
Characteristics organismpart: skeletal muscle
Sex: male
age: 77 years
phenotype: caucasian
material type: total_RNA
libraryprep: mRNA-Seq
biosourceprovider: Human skeletal muscle total RNA, lot 046P021205086A
Extracted molecule total RNA
Extraction protocol nucleic_acid_extraction | 1ug of total RNA was subjected to two rounds of oligo-dT beads binding. Purified mRNA was fragmented and random primed for cDNA synthesis. cDNA fragements were end repared, added dATP, and liagted to illumina pair end sample prep adaptor. The ligated material was amplified by PCR for 15 cycles, and used for illumina sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description 50bp_PE_mRNA_Seq_FCA_s_5_1_sequence
2x50 paired end mRNA-seq READ1
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
Data processing processed data not provided
 
Submission date Jul 12, 2011
Last update date May 15, 2019
Organization European Bioinformatics Institute
E-mail(s) miamexpress@ebi.ac.uk
Lab ArrayExpress
Street address Wellcome Trust Genome Campus
City Hinxton
State/province Cambridgeshire
ZIP/Postal code CB10 1SD
Country United Kingdom
 
Platform ID GPL11154
Series (2)
GSE30611 [E-MTAB-513] Illumina Human Body Map 2.0 Project
GSE49417 The conserved organization of the human and mouse transcriptomes
Relations
Reanalyzed by GSE97211
SRA ERX011212

Supplementary data files not provided
SRA Run SelectorHelp
Processed data not provided for this record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap