|
| Status |
Public on May 16, 2024 |
| Title |
NK_D2_R |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC
subject: donor2
cell type: primary NK cells
disease state: healthy
cell population: Responsive
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Primary NK cells after sorting were processed for RNA isolation. Isolation was performed based on the Quick-RNA Microprep (Cat no. R1050 & R1051; ZymoResearch) according to the manufacturers’ protocol.
RNA libraries were prepared using Illumina Stranded RNA Kit following manufacter's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The RNA sequencing data was trimmed using Trim_galore (version 0.5.0) and aligned using STAR (v2.7.3a) with default parameters. The ENSEMBL human hg38 build and its corresponding GTF were used to build the STAR reference database. The number of reads per gene was quantified using HTSeq-Count (v0.6.1p1).
Assembly: hg38
Supplementary files format and content: tab-delimited file with gene name and raw counts
|
| |
|
| Submission date |
Nov 20, 2023 |
| Last update date |
May 16, 2024 |
| Contact name |
Tirza T Doniger |
| E-mail(s) |
tirza.doniger@biu.ac.il
|
| Phone |
+972 3 531 8642
|
| Organization name |
Bar Ilan University
|
| Department |
The Mina and Everard Faculty of Life Sciences
|
| Street address |
Bar Ilan University
|
| City |
Ramat Gan |
| State/province |
Not US or Canada |
| ZIP/Postal code |
5290002 |
| Country |
Israel |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE248228 |
Profiling and Rewiring Dysfunctional NK cells to Restore Anti-Tumor Activity |
|
| Relations |
| BioSample |
SAMN38329728 |
| SRA |
SRX22581637 |
