|
| Status |
Public on Dec 18, 2023 |
| Title |
BAB14686 Proband |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Whole Blood Extracted Genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from peripheral blood with a Puregene kit (Gentra Systems), following the manufacturer’s protocol
|
| Label |
Cy5
|
| Label protocol |
1.2 ug of genomic patient and reference DNA were digested with AluI (5 U) and RsaI (5 U) (Promega, R6281 and R6371) for 2 hr at 37°C. Labeling reactions were performed according to the manufacturer’s instructions (BioPrime Array CGH Genomic Labeling Module,Invitrogen Corporation, #18095-012; Perkin-Elmer Las Inc. Cyanine Smart Pack dCTP,
|
| |
|
| Channel 2 |
| Source name |
BAB14686
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
sample type: Reference Sample
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from peripheral blood with a Puregene kit (Gentra Systems), following the manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
1.2 ug of genomic patient and reference DNA were digested with AluI (5 U) and RsaI (5 U) (Promega, R6281 and R6371) for 2 hr at 37°C. Labeling reactions were performed according to the manufacturer’s instructions (BioPrime Array CGH Genomic Labeling Module,Invitrogen Corporation, #18095-012; Perkin-Elmer Las Inc. Cyanine Smart Pack dCTP,
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed following the manufacturer’s protocol.
|
| Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 12.0)
|
| Data processing |
Agilent Feature Extraction Software (version 12.0) was used for background subtraction and normalization.
|
| |
|
| Submission date |
Dec 18, 2023 |
| Last update date |
Dec 18, 2023 |
| Contact name |
Christopher Grochowski |
| E-mail(s) |
Christopher.Grochowski@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77006 |
| Country |
USA |
| |
|
| Platform ID |
GPL34011 |
| Series (1) |
| GSE250451 |
Break-induced replication underlies formation of inverted triplications and generates unexpected diversity in haplotype structures |
|
