|
Status |
Public on Jan 13, 2024 |
Title |
FOS_EGR1-clone_2-utr2-R2 |
Sample type |
SRA |
|
|
Source name |
HEK-RAF-ER
|
Organism |
Homo sapiens |
Characteristics |
cell line: HEK-RAF-ER genotype: FOS_EGR1 clone: clone 2 treatment: none time: utr2 replicate: R2
|
Treatment protocol |
Cells were treated with tamoxifen (4OHT) to activate RAF activity
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was extracted using Qiagen RNAeasy kit following manufacturers recommendation, without removal of DNA Library was constructed using QuantSeq 3’ mRNA-Seq V2 Library Prep Kit V2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
poly-A sequences and adapters are trimmed with bbduk Sequences are aligned using STAR aligner Assembly: GRCh38 Supplementary files format and content: counts of reads per Gene
|
|
|
Submission date |
Dec 19, 2023 |
Last update date |
Jan 13, 2024 |
Contact name |
Nils Blüthgen |
E-mail(s) |
nils.bluethgen@charite.de
|
Organization name |
Charite Universitätsmedizin Berlin
|
Department |
Institute of Pathology
|
Street address |
Chariteplatz 1
|
City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE250532 |
Effects of kockouts of EGR1, FOS and their combination on the MAPK induced transcriptome [RNA_KO_clones] |
GSE250559 |
MAPK |
|
Relations |
BioSample |
SAMN38932568 |
SRA |
SRX22970330 |