|
Status |
Public on Jan 13, 2024 |
Title |
run1a_05h_8h |
Sample type |
SRA |
|
|
Source name |
HEK-RAF-ER
|
Organism |
Homo sapiens |
Characteristics |
replicate: R1 cell line: HEK-RAF-ER time of_measurement: 8h pulse length: 0.5h treatment: tamoxifen (4OHT)
|
Treatment protocol |
Cells were treated with tamoxifen to induce RAF/MAPK signalling for various times (0.5-8h) and MAPK activity was blocked using AZD6422 inhibitor
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was extracted with Trizol. Sequencing libraries were prepared using Illumina TruSeq mRNA Library Prep Kit v2 following manufacturers recommendation
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Data was processed using snakemake-workflows rna-seq-star-deseq2 pipeline, v1.2.0 Assembly: GRCh38 Supplementary files format and content: counts resulting from the star aligner
|
|
|
Submission date |
Dec 19, 2023 |
Last update date |
Jan 13, 2024 |
Contact name |
Nils Blüthgen |
E-mail(s) |
nils.bluethgen@charite.de
|
Organization name |
Charite Universitätsmedizin Berlin
|
Department |
Institute of Pathology
|
Street address |
Chariteplatz 1
|
City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
|
|
Platform ID |
GPL11154 |
Series (2) |
GSE250533 |
Transcriptome analysis of pulsed activation of MAPK signaling in HEK-RAF-ER cells [RNA_seq_pulse_time_series] |
GSE250559 |
MAPK |
|
Relations |
BioSample |
SAMN38932332 |
SRA |
SRX22970128 |