|
| Status |
Public on Jan 13, 2024 |
| Title |
run2a_8h_1h |
| Sample type |
SRA |
| |
|
| Source name |
HEK-RAF-ER
|
| Organism |
Homo sapiens |
| Characteristics |
replicate: R2
cell line: HEK-RAF-ER
time of_measurement: 1h
pulse length: 8h
treatment: tamoxifen (4OHT)
|
| Treatment protocol |
Cells were treated with tamoxifen to induce RAF/MAPK signalling for various times (0.5-8h) and MAPK activity was blocked using AZD6422 inhibitor
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted with Trizol.
Sequencing libraries were prepared using Illumina TruSeq mRNA Library Prep Kit v2 following manufacturers recommendation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Data was processed using snakemake-workflows rna-seq-star-deseq2 pipeline, v1.2.0
Assembly: GRCh38
Supplementary files format and content: counts resulting from the star aligner
|
| |
|
| Submission date |
Dec 19, 2023 |
| Last update date |
Jan 13, 2024 |
| Contact name |
Nils Blüthgen |
| E-mail(s) |
nils.bluethgen@charite.de
|
| Organization name |
Charite Universitätsmedizin Berlin
|
| Department |
Institute of Pathology
|
| Street address |
Chariteplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE250533 |
Transcriptome analysis of pulsed activation of MAPK signaling in HEK-RAF-ER cells [RNA_seq_pulse_time_series] |
| GSE250559 |
MAPK |
|
| Relations |
| BioSample |
SAMN38932315 |
| SRA |
SRX22970145 |
