|
| Status |
Public on Apr 24, 2024 |
| Title |
Patients No2-after |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver biopsied tissue
disease state: metabolic dysfunction-associated steatohepatitis (MASH)
age: 29
condition: after L-carnitine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNAqueousTM-Micro Total RNA Isolation Kit AM1931 (Thermo Fisher Scientific K.K., Yokohama, Japan) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Aliquots of total RNA (50 ng) isolated from biopsied liver tissues were subjected to amplification using the Ovation Pico WTA System V2 3302-12 (AS ONE, Japan) according to the manufacturer’s instructions. Approximately 10 μg of cDNA was amplified from 50 ng of total RNA, and 5 μg of cDNA was used for fragmentation and biotin labeling using the FL-Ovation cDNA Biotin Module V2 (NuGen) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Biotin-labeled cDNA was suspended in 220 ul of hybridization cocktail (NuGen) and 200 ul was used for hybridization to the Affymetrix Human 133 Plus 2.0 microarray chip containing 54,675 probes.GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
After stringent washing, the microarray chips were stained with streptavidin-phycoerythrin and probe hybridization was determined using a GeneChip Scanner 3000 (Affymetrix).
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. If the detection call contained 'Absent', the probe set was removed from analysis.
|
| |
|
| Submission date |
Mar 19, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Masao Honda |
| E-mail(s) |
mhondag@gmail.com
|
| Phone |
0762652243
|
| Organization name |
Kanazawa University
|
| Department |
Gastroenterology
|
| Street address |
Takara-Machi 13-1
|
| City |
Kanazawa |
| ZIP/Postal code |
920-8641 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE261938 |
Potential utility of L-carnitine for preventing liver tumors derived from metabolic dysfunction-associated steatohepatitis |
|
