|
| Status |
Public on Apr 24, 2024 |
| Title |
shRPL35A_02 |
| Sample type |
RNA |
| |
|
| Source name |
BC457-2_(PrimeView)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H1299
treatment: infected with shRPL35A
|
| Treatment protocol |
NCI-H1299 cells in logarithmic growth phase were infected by adding 20 μL 1×108 TU/mL shCtrl or shRPL35A lentivirus, culturing in 1640 medium with 10% FBS in a 6-well dish with 2×105 cells per well.
|
| Growth protocol |
NCI-H1299 cells were cultured in 1640 medium containing 10% fetal bovine serum (FBS) in cell incubator with 5% CO2 at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
3’ IVT Biotin Label
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip primeview human PathArrayTM.
|
| Scan protocol |
Affymetrix human GeneChip PrimeView and the outcomes were scanned by Affymetrix Scanner 3000.
|
| Data processing |
The statistical significance of raw data was completed by a Welch t-test with Benjamini-Hochberg FDR.
|
| |
|
| Submission date |
Apr 19, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Liqin Yan |
| E-mail(s) |
syyyylq@d.sxmu.edu.cn, YLQ691450@163.com
|
| Phone |
+8613834691450
|
| Organization name |
Shanxi Medical University
|
| Street address |
Jiefang Road
|
| City |
Taiyuan |
| ZIP/Postal code |
030000 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE264428 |
Gene expression in NCI-H1299 cells infected with shCtrl and shRPL35A |
|
