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| Status |
Public on Apr 27, 2024 |
| Title |
Neural stem cells_TFQA treatment_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Brain of 6-8 weeks old adult male ICR mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Neural stem cells
tissue: Brain
strain: ICR
Sex: male
treatment: TFQA treatment
|
| Treatment protocol |
Neural stem cells were randomly assigned to five group: control, 1 uM TCQA- treatment, 5 uM TCQA- treatment, 1 uM TFQA- treatment and 5 uM TFQA- treatment. The NSCs were seeded in 6-well plates (BD Falcon, USA) at the density of 100000 cells/well and were incubated at 37 ºC. NSCs were treated with differentiation medium without or with 1 and 5 μM of TCQA and TFQA respectively for 72 h.
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| Growth protocol |
6-8 weeks old adult male ICR mice (Charles River, Japan) were used for the experiment.NSCs were isolated and collected from ICR mice brains using the adult brain dissociation kit (Miltenyi Biotec, Germany), Anti-Prominin-1 MicroBeads (Miltenyi Biotec, Germany) and the autoMACS pro separator (Miltenyi Biotec, Germany). The NSCs were cultured at 37 ℃ in a 95% air / 5% CO2 humidified Heracell™ VIOS 160i CO2 incubator (Thermo Fisher Scientific, USA). The medium was changed every three days to keep cells survival.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from neural stem cells using the ISOGEN kit (Nippon Gene Co. Ltd., Tokyo, Japan), following the manufacturer's instructions. Total RNA concentration was determined using the NanoDrop One / OneC (Thermo Fisher Scientific, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 9.1 ug total RNA.
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| |
|
| Hybridization protocol |
Following fragmentation, 100 ng of cRNA were hybridized for 16 h at 45 ºC on GeneChip Mouse Genome Array. Gene Chips were washed and stained in the Gene Atlas Fluidics Station 400.
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| Scan protocol |
Gene Chips were scanned using the GeneChip Scanner 3000 (thermo Fisher Science, Japan).
|
| Description |
Gene expression data from neural stem cells of adult mice
|
| Data processing |
The raw CEL data were normalized using the transcriptome analysis cosole (TAC) software ver.4.0.1 following the robust multichip average (RMA) algorithm (Thermo Fisher Scientific, Japan)
%result_name=Analysis_9
%array_type=Clariom_S_Mouse
%annotation=Clariom_S_Mouse.r1.na36.mm10.a1.transcript.csv
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| |
|
| Submission date |
Apr 22, 2024 |
| Last update date |
Apr 27, 2024 |
| Contact name |
Hongyu Lin |
| E-mail(s) |
linhongyu1hk@gmail.com
|
| Phone |
+8615521299752
|
| Organization name |
University of Tsukuba
|
| Department |
Life and Earth Sciences
|
| Lab |
ISODA Lab.
|
| Street address |
Tennodai 1-1-1
|
| City |
Tsukuba City |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE264538 |
Expression data from neural stem cells of adult mice brains in TCQA- and TFQA- treatments |
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