|
Status |
Public on Jun 25, 2012 |
Title |
bzr1-1D_PAC_PPZ |
Sample type |
SRA |
|
|
Source name |
bzr1-1D_PAC_PPZ
|
Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Col-0 genotype/variation: bzr1-1D tissue: Hypocotyl
|
Treatment protocol |
Added 5 ml liquid solution with 10 μM GA3 or mock to the plate, incubated 5 min and pooled out the liqid and keep the plate another 12 hr.
|
Growth protocol |
Wild type Arabidopsis and bzr1-1D were grown in media containing 1 μM PAC and 0 or 2 μM PPZ for 4.5 days in dark
|
Extracted molecule |
total RNA |
Extraction protocol |
Libraries were constructed with barcodes using TruSeqTM RNA Sample Preparation Kit (Ilumina)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
The sequence reads were mapped to the Arabidopsis genome using Tophat software, and differential gene expression was analyzed using DeSeq software.
|
|
|
Submission date |
Jan 30, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Mingyi Bai |
Organization name |
Shandong University
|
Department |
Life of Scienec
|
Lab |
Bai
|
Street address |
27 Shandananlu
|
City |
Jinan |
State/province |
Shandong |
ZIP/Postal code |
250100 |
Country |
China |
|
|
Platform ID |
GPL13222 |
Series (1) |
GSE35408 |
Brassinosteroid, gibberellin, and phytochrome impinge on a common transcription module in Arabidopsis |
|
Relations |
SRA |
SRX118087 |
BioSample |
SAMN00779667 |