|
| Status |
Public on Apr 25, 2012 |
| Title |
[E-MTAB-935] polyA+_brain_6120217, Batch_7_brain |
| Sample type |
SRA |
| |
|
| Source name |
polyA+_brain_6120217
|
| Organism |
Homo sapiens |
| Characteristics |
material type: organism_part
organismpart: brain
Sex: male
biosourcetype: frozen_sample
biosourceprovider: Clontech
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
nucleic_acid_extraction | RNA from tissues was isolated by a modified guanidium thiocynate methods followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. Selected targets were then amplified by RT-PCR
sequencing | Aliquots of up to 5000 RT-PCRs reactions were pooled together and the sequencing library was prepared with no fragmentation using the TruSeq DNA protocol. DNA fragments were sequenced on a Illumina HiSeq2000 sequencer and 2 multiplexed libraries were loaded per lane. The sequencing was performed at the University of Lausanne, Lausanne, Switzerland.
bioassay_data_transformation |
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Performer: University of Lausanne, Switzerland
|
| Data processing |
processed data not provided
|
| |
|
| Submission date |
Apr 25, 2012 |
| Last update date |
May 15, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
|
| Lab |
ArrayExpress
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE37592 |
[E-MTAB-935] GENCODE PCR-Seq Batch VII |
|
| Relations |
| SRA |
ERX046919 |
