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Sample GSM923038 Query DataSets for GSM923038
Status Public on Apr 25, 2012
Title [E-MTAB-935] polyA+_testis_7060321, Batch_7_testis
Sample type SRA
 
Source name polyA+_testis_7060321
Organism Homo sapiens
Characteristics material type: organism_part
organismpart: testis
Sex: male
biosourcetype: frozen_sample
biosourceprovider: Clontech
Extracted molecule polyA RNA
Extraction protocol nucleic_acid_extraction | RNA from tissues was isolated by a modified guanidium thiocynate methods followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. Selected targets were then amplified by RT-PCR
sequencing | Aliquots of up to 5000 RT-PCRs reactions were pooled together and the sequencing library was prepared with no fragmentation using the TruSeq DNA protocol. DNA fragments were sequenced on a Illumina HiSeq2000 sequencer and 2 multiplexed libraries were loaded per lane. The sequencing was performed at the University of Lausanne, Lausanne, Switzerland.
bioassay_data_transformation |
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description Performer: University of Lausanne, Switzerland
Data processing processed data not provided
 
Submission date Apr 25, 2012
Last update date May 15, 2019
Organization European Bioinformatics Institute
E-mail(s) miamexpress@ebi.ac.uk
Lab ArrayExpress
Street address Wellcome Trust Genome Campus
City Hinxton
State/province Cambridgeshire
ZIP/Postal code CB10 1SD
Country United Kingdom
 
Platform ID GPL11154
Series (1)
GSE37592 [E-MTAB-935] GENCODE PCR-Seq Batch VII
Relations
SRA ERX046922

Supplementary data files not provided
SRA Run SelectorHelp
Processed data not provided for this record
Raw data are available in SRA

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