|
| Status |
Public on Nov 24, 2014 |
| Title |
Patient 6_NAT |
| Sample type |
RNA |
| |
|
| Source name |
FFPE tissue specimen
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal adjacent breast
lymph node positive: N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 2mm punch biopsies of FFPE tissue using the miRNeasy FFPE kit (Qiagen, Doncaster, VIC, Australia).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was dephosphorylated and directly labelled with Cy3 using the miRNA Complete Labelling and Hyb Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Labelled RNA was hybridised to Human miRNA microarrays (Sanger Release 14.0) according to the manufacturers’ instructions (Agilent Technologies)
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent High-resolution C Scanner using one color scan setting for miRNA array slides.
|
| Description |
miRNA expression
|
| Data processing |
Data from 15,000 probe features representing 904 unique miRNAs was extracted using Agilent Feature Extraction software (v10.7.3.1) using default parameters (protocol miRNA_107_09 and Grid: 029297_D_F_20100712) and converted to background subtracted signal intensities.
|
| |
|
| Submission date |
May 23, 2012 |
| Last update date |
Nov 24, 2014 |
| Contact name |
Kelly Kiejda |
| E-mail(s) |
Kelly.Kiejda@newcastle.edu.au
|
| Organization name |
University of Newcastle
|
| Street address |
HMRI
|
| City |
New Lambton Heights |
| State/province |
NSW |
| ZIP/Postal code |
2305 |
| Country |
Australia |
| |
|
| Platform ID |
GPL14943 |
| Series (1) |
| GSE38167 |
Decreased expression of key tumour suppressor microRNAs is associated with lymph node metastases in triple negative breast cancer |
|
