|
| Status |
Public on Jun 25, 2012 |
| Title |
Col_PAC GA repeat |
| Sample type |
SRA |
| |
|
| Source name |
Col_PAC GA
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype/variation: wild type
tissue: Hypocotyl
|
| Treatment protocol |
Added 5 ml liquid solution with 10 μM GA3 or mock to the plate, incubated 5 min and pooled out the liqid and keep the plate another 12 hr.
|
| Growth protocol |
Wild type Arabidopsis and bzr1-1D were grown in media containing 1 μM PAC and 0 or 2 μM PPZ for 4.5 days in dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Libraries were constructed with barcodes using TruSeqTM RNA Sample Preparation Kit (Ilumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
The sequence reads were mapped to the Arabidopsis genome using Tophat software, and differential gene expression was analyzed using DeSeq software.
|
| |
|
| Submission date |
Jun 25, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Mingyi Bai |
| Organization name |
Shandong University
|
| Department |
Life of Scienec
|
| Lab |
Bai
|
| Street address |
27 Shandananlu
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250100 |
| Country |
China |
| |
|
| Platform ID |
GPL13222 |
| Series (1) |
| GSE35408 |
Brassinosteroid, gibberellin, and phytochrome impinge on a common transcription module in Arabidopsis |
|
| Relations |
| SRA |
SRX155845 |
| BioSample |
SAMN01057575 |
