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Sample GSM972939 Query DataSets for GSM972939
Status Public on Jul 23, 2013
Title CD4+ T cells, baseline, biological replicate 3
Sample type RNA
 
Source name CD4+ T cells, baseline
Organism Homo sapiens
Characteristics diagnosis: sarcoidosis
cell type: CD4+ T cells
Treatment protocol Participants received the oral CLEAR regimen: Levofloxacin 750 mg on day 1, then 500 mg daily, Ethambutol 1200 mg daily, Azithromycin 500 mg on day 1, then 250 mg daily, and Rifampin 300 mg daily, or a comparative placebo regimen, for a total of 8 weeks, with a 180 day follow-up.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3 labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
 
Hybridization protocol Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microRNA array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
Description C24-4_710
Data processing The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters.
 
Submission date Jul 24, 2012
Last update date Jul 23, 2013
Contact name Kusum Vijay Pandit
E-mail(s) panditkusum@yahoo.com
Phone 412-624-9046
Organization name University of Pittsburgh
Department Medicine-PACCM
Street address 200, Lothrop street, W1200-25A Biomedical Science Tower
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL14550
Series (1)
GSE39606 Gene expression data in CD4+ and CD8+ cells in sarcoidosis patients following antimycobacterial therapy

Data table header descriptions
ID_REF
VALUE Cyclic lowess normalized signal intensity

Data table
ID_REF VALUE
A_23_P100001 3.5397475
A_23_P100022 3.7744617
A_23_P100056 5.1372952
A_23_P100315 7.855943
A_23_P100326 10.398691
A_23_P100499 7.1726847
A_23_P100764 9.85506
A_23_P100963 7.31995
A_23_P101048 2.5762153
A_23_P101073 3.0844452
A_23_P101297 7.9458437
A_23_P101332 10.01362
A_23_P10135 8.77105
A_23_P101480 5.4271803
A_23_P101516 5.7312927
A_23_P101532 16.533342
A_23_P10156 8.453883
A_23_P101642 3.4522507
A_23_P101699 6.350028
A_23_P10172 2.544364

Total number of rows: 29271

Table truncated, full table size 648 Kbytes.




Supplementary file Size Download File type/resource
GSM972939_C24-4_710.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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